Egr1 mediates the effect of insulin on leptin transcription in adipocytes.
J Biol Chem
; 294(15): 5784-5789, 2019 04 12.
Article
em En
| MEDLINE
| ID: mdl-30846562
ABSTRACT
In mammals, leptin production in adipocytes is up-regulated by feeding and insulin. Although this regulatory connection is central to all physiological effects of leptin, its molecular mechanism remains unknown. Here, we show that the transcription factor early growth response 1, Egr1, is rapidly but transiently induced by insulin in adipose cells both in vitro and in vivo, and its induction is followed by an increase in leptin transcription. ChIP and luciferase assays demonstrate that Egr1 directly binds to and activates the leptin promoter. Interestingly, the lipid droplet protein FSP27 may work as a co-factor for Egr1 in regulating leptin expression. By using siRNA-mediated knockout of Egr1 along with its overexpression in adipocytes, we demonstrate that Egr1 is both necessary and sufficient for the stimulatory effect of insulin on leptin transcription.
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Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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Adipócitos
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Elementos de Resposta
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Leptina
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Proteína 1 de Resposta de Crescimento Precoce
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Insulina
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article