Ezh1 arises from Ezh2 gene duplication but its function is not required for zebrafish development.
Sci Rep
; 9(1): 4319, 2019 03 13.
Article
em En
| MEDLINE
| ID: mdl-30867490
ABSTRACT
Trimethylation on H3K27 mediated by Polycomb Repressive Complex 2 (PRC2) is required to control gene repression programs involved in development, regulation of tissue homeostasis or maintenance and lineage specification of stem cells. In Drosophila, the PRC2 catalytic subunit is the single protein E(z), while in mammals this function is fulfilled by two proteins, Ezh1 and Ezh2. Based on database searches, we propose that Ezh1 arose from an Ezh2 gene duplication that has occurred in the common ancestor to elasmobranchs and bony vertebrates. Expression studies in zebrafish using in situ hybridization and RT-PCR followed by the sequencing of the amplicon revealed that ezh1 mRNAs are maternally deposited. Then, ezh1 transcripts are ubiquitously distributed in the entire embryo at 24 hpf and become more restricted to anterior part of the embryo at later developmental stages. To unveil the function of ezh1 in zebrafish, a mutant line was generated using the TALEN technology. Ezh1-deficient mutant fish are viable and fertile, but the loss of ezh1 function is responsible for the earlier death of ezh2 mutant larvae indicating that ezh1 contributes to zebrafish development in absence of zygotic ezh2 gene function. Furthermore, we show that presence of ezh1 transcripts from the maternal origin accounts for the delayed lethality of ezh2-deficient larvae.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
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Duplicação Gênica
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Proteínas de Peixe-Zebra
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Complexo Repressor Polycomb 2
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Proteína Potenciadora do Homólogo 2 de Zeste
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article