Your browser doesn't support javascript.
loading
An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime.
Bett, Bosibori; Gollasch, Stephanie; Moore, Andy; Harding, Robert; Higgins, Thomas J V.
Afiliação
  • Bett B; CSIRO Agriculture and Food, Canberra, ACT, Australia.
  • Gollasch S; Centre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, QLD, Australia.
  • Moore A; Biotechnology Centre, Kenya Agricultural & Livestock Research Organisation, Nairobi, Kenya.
  • Harding R; CSIRO Agriculture and Food, Canberra, ACT, Australia.
  • Higgins TJV; CSIRO Agriculture and Food, Canberra, ACT, Australia.
Front Plant Sci ; 10: 219, 2019.
Article em En | MEDLINE | ID: mdl-30873198
An improved cowpea transformation method utilizing Agrobacterium-mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kanamycin and geneticin. The method was reproducible and led to the recovery of independent fertile transgenic plants in the greenhouse at a level of about one per cent of starting explants. A transgene encoding an insecticidal protein from Bacillus thuringiensis was used to demonstrate the efficacy of the system.
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2019 Tipo de documento: Article