Complex N-glycan promotes CD133 mono-ubiquitination and secretion.
FEBS Lett
; 593(7): 719-731, 2019 04.
Article
em En
| MEDLINE
| ID: mdl-30873590
ABSTRACT
CD133 is a widely used cell surface marker of cancer stem cells that plays an important role in tumor initiation and metastasis. Increasing evidence shows that CD133 is secreted to the extracellular space. However, the underlying mechanisms of CD133 secretion remain largely unknown. In this study, we report that secreted CD133 has a complex-type N-glycosylation and is modified by beta1,6GlcNAc N-glycan. We found that inhibition of CD133 complex-type N-glycosylation by swainsonine does not affect the membrane localization of CD133, but significantly reduces CD133 secretion and promotes its accumulation in early endosomes. Moreover, swainsonine reduces CD133 secretion by reducing its mono-ubiquitination and inhibiting the interaction between CD133 and Tsg101. These findings reveal a new mechanism of glycosylation-dependent secretion of CD133.
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MEDLINE
Assunto principal:
Fatores de Transcrição
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Biomarcadores Tumorais
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Proteínas de Ligação a DNA
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Complexos Endossomais de Distribuição Requeridos para Transporte
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Antígeno AC133
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Neoplasias
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article