Antioxidant and immunomodulatory activity induced by stevioside in liver damage: In vivo, in vitro and in silico assays.
Life Sci
; 224: 187-196, 2019 May 01.
Article
em En
| MEDLINE
| ID: mdl-30890404
ABSTRACT
AIMS:
Stevioside is a diterpenoid obtained from the leaves of Stevia rebaudiana (Bertoni) that exhibits antioxidant, antifibrotic, antiglycemic and anticancer properties. Therefore, we aimed to study whether stevioside has beneficial effects in liver injury induced by long-term thioacetamide (TAA) administration and investigated the possible underlying molecular mechanism using in vivo, in vitro and in silico approaches. MAINMETHODS:
Liver injury was induced in male Wistar rats by TAA administration (200â¯mg/kg), intraperitoneally, three times per week. Rats received saline or stevioside (20â¯mg/kg) twice daily intraperitoneally. In addition, cocultures were incubated with either lipopolysaccharide or ethanol. Liver injury, antioxidant and immunological responses were evaluated. KEYFINDINGS:
Chronic TAA administration induced significant liver damage. In addition, TAA upregulated the protein expression of nuclear factor (NF)-κB, thus increasing the expression of proinflammatory cytokines and decreasing the antioxidant capacity of the liver through downregulation of nuclear erythroid factor 2 (Nrf2). Notably, stevioside administration prevented all of these changes. In vitro, stevioside prevented the upregulation of several genes implicated in liver inflammation when cocultured cells were incubated with lipopolysaccharide or ethanol. In silico assays using tumor necrosis factor receptor (TNFR)-1 and Toll-like receptor (TLR)-4-MD2 demonstrated that stevioside docks with TNFR1 and TLR4-MD2, thus promoting an antagonistic action against this proinflammatory mediator.SIGNIFICANCE:
Collectively, these data suggest that stevioside prevented liver damage through antioxidant activity by upregulating Nrf2 and immunomodulatory activity by blocking NF-κB signaling.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Edulcorantes
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Diterpenos do Tipo Caurano
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Doença Hepática Induzida por Substâncias e Drogas
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Glucosídeos
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Fatores Imunológicos
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Antioxidantes
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article