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Human Antibody Bispecifics through Phage Display Selection.
Luthra, Ansha; Langley, David B; Schofield, Peter; Jackson, Jennifer; Abdelatti, Mahmoud; Rouet, Romain; Nevoltris, Damien; Mazigi, Ohan; Crossett, Ben; Christie, Mary; Christ, Daniel.
Afiliação
  • Luthra A; Garvan Institute of Medical Research , 384 Victoria Road , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Langley DB; Faculty of Medicine, St Vincent's Clinical School , The University of New South Wales , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Schofield P; Garvan Institute of Medical Research , 384 Victoria Road , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Jackson J; Garvan Institute of Medical Research , 384 Victoria Road , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Abdelatti M; Faculty of Medicine, St Vincent's Clinical School , The University of New South Wales , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Rouet R; Garvan Institute of Medical Research , 384 Victoria Road , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Nevoltris D; Garvan Institute of Medical Research , 384 Victoria Road , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Mazigi O; Garvan Institute of Medical Research , 384 Victoria Road , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Crossett B; Garvan Institute of Medical Research , 384 Victoria Road , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Christie M; Garvan Institute of Medical Research , 384 Victoria Road , Darlinghurst, Sydney , New South Wales 2010 , Australia.
  • Christ D; Faculty of Medicine, St Vincent's Clinical School , The University of New South Wales , Darlinghurst, Sydney , New South Wales 2010 , Australia.
Biochemistry ; 58(13): 1701-1704, 2019 04 02.
Article em En | MEDLINE | ID: mdl-30900875
ABSTRACT
We developed a repertoire approach to generate human antibody bispecifics. Using phage display selection of antibody heavy chains in the presence of a competitor light chain and providing a cognate light chain with an affinity handle, we identified mutations that prevent heavy/light chain mispairing. The strategy allows for the selection of human antibody chains that autonomously assemble into bispecifics.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Anticorpos Biespecíficos / Biblioteca de Peptídeos Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Anticorpos Biespecíficos / Biblioteca de Peptídeos Idioma: En Ano de publicação: 2019 Tipo de documento: Article