Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells.
J Vis Exp
; (145)2019 03 06.
Article
em En
| MEDLINE
| ID: mdl-30907887
ABSTRACT
The endothelial cells of skeletal muscle capillaries (muscle microvascular endothelial cells, MMEC) build up the barrier between blood stream and skeletal muscles regulating the exchange of fluids and nutrients as well as the immune response against infectious agents by controlling immune cell migration. For these functions, MMEC form a functional "myovascular unit" (MVU), with further cell types, such as fibroblasts, pericytes and skeletal muscle cells. Consequently, a dysfunction of MMEC and therefore the MVU contributes to a vast variety of myopathies. However, regulatory mechanisms of MMEC in health and disease remain insufficiently understood and their elucidation precedes more specific treatments for myopathies. The isolation and in-depth investigation of primary MMEC functions in the context of the MVU might facilitate a better understanding of these processes. This article provides a protocol to isolate primary murine MMEC of the skeletal muscle by mechanical and enzymatic dissociation including purification and culture maintenance steps.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Músculo Esquelético
/
Células Endoteliais
/
Microvasos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article