Effects of supplementation of Tris-egg yolk extender with royal jelly on chilled and frozen-thawed ram semen characteristics.
Cryobiology
; 88: 75-80, 2019 06.
Article
em En
| MEDLINE
| ID: mdl-30910554
ABSTRACT
This study was conducted to examine the effect of supplementation of Tris-egg yolk extender with lyophilized royal jelly (RJ) on chilled and frozen-thawed ram semen parameters. Ejaculates were collected by artificial vagina from 4 mature rams, twice a week for 4 weeks. Only samples with motility of ≥70% were included, pooled and divided into four equal parts and then diluted in extenders with various concentrations of RJ (0, 1, 3 and 5%, vol/vol) to a final concentration of 200â¯×â¯106 sperm/mL and was incubated at 37⯰C for 30â¯min and were subsequently evaluated. After equilibration of extended semen for 2â¯hâ¯at 4⯰C, some semen samples were packed in 0.25â¯mL plastic straws. Then, the straws were frozen in the liquid nitrogen vapor phase for 15â¯min and stored at -196⯰C in liquid nitrogen. The frozen straws were thawed in warm water (37⯰C) for 30â¯s and evaluated; whereas, other semen samples were stored in the refrigerator (4⯰C) up to 7 days. The chilled samples were kept in water bath (37⯰C) for 5â¯min and then were evaluated. After dilution, the lowest and highest sperm total abnormality was recorded in 3 and 5% RJ supplemented groups, respectively (Pâ¯<â¯0.05). The chilled sperm total motility and membrane integrity were significantly (Pâ¯<â¯0.05) higher in 3% than those in 0% and 5% RJ supplemented groups. The chilled sperm progressive motility and viability was significantly (Pâ¯<â¯0.05) higher in 1 and 3% than those in 0 and 5% RJ supplemented groups. The frozen-thawed sperm total motility, progressive motility, membrane integrity and viability were significantly higher in 3% RJ supplemented group (Pâ¯<â¯0.05). In conclusion, supplementation of Tris-egg yolk extender with 3% lyophilized RJ had a protective effect on chilled and cryopreserved ram spermatozoa.
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MEDLINE
Assunto principal:
Sêmen
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Preservação do Sêmen
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Criopreservação
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Crioprotetores
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Ácidos Graxos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article