T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model.
J Neuroimmunol
; 332: 49-56, 2019 07 15.
Article
em En
| MEDLINE
| ID: mdl-30933850
ABSTRACT
The experimental autoimmune encephalomyelitis (EAE) model is indispensable for autoimmunity research, but model-specific T cell dynamics are sparsely studied. We used next-generation immunosequencing across lymphoid organs, blood and spinal cord in response to immunization with myelin basic protein (MBP) to study T cell repertoires and migration patterns. Surprisingly, most spinal cord T cells were unique to the individual animal despite the existence of shared MBP-specific clones, suggesting a previously underestimated T cell diversity. Almost complete emigration of pathogenic clones from blood to spinal cord indicates that blood is not a suitable compartment to study EAE-mediating T cells.
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MEDLINE
Assunto principal:
Subpopulações de Linfócitos T
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Especificidade do Receptor de Antígeno de Linfócitos T
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Encefalomielite Autoimune Experimental
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Seleção Clonal Mediada por Antígeno
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article