Active PI3K abrogates central tolerance in high-avidity autoreactive B cells.
J Exp Med
; 216(5): 1135-1153, 2019 05 06.
Article
em En
| MEDLINE
| ID: mdl-30948496
ABSTRACT
Autoreactive B cells that bind self-antigen with high avidity in the bone marrow undergo mechanisms of central tolerance that prevent their entry into the peripheral B cell population. These mechanisms are breached in many autoimmune patients, increasing their risk of B cell-mediated autoimmune diseases. Resolving the molecular pathways that can break central B cell tolerance could therefore provide avenues to diminish autoimmunity. Here, we show that B cell-intrinsic expression of a constitutively active form of PI3K-P110α by high-avidity autoreactive B cells of mice completely abrogates central B cell tolerance and further promotes these cells to escape from the bone marrow, differentiate in peripheral tissue, and undergo activation in response to self-antigen. Upon stimulation with T cell help factors, these B cells secrete antibodies in vitro but remain unable to secrete autoantibodies in vivo. Overall, our data demonstrate that activation of the PI3K pathway leads high-avidity autoreactive B cells to breach central, but not late, stages of peripheral tolerance.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Autoantígenos
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Linfócitos B
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Autoimunidade
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Classe Ia de Fosfatidilinositol 3-Quinase
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Tolerância Central
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article