Paracoccus haematequi sp. nov., isolated from horse blood.
Int J Syst Evol Microbiol
; 69(6): 1682-1688, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30958257
ABSTRACT
Two slightly orange-pigmented, oxidase-positive bacterial strains (M1-83T and M2-116), isolated from horse blood collected during slaughter in Giessen, Germany, were studied in a polyphasic taxonomic approach. Cells of the isolates were coccoid and stained Gram-negative. The two strains shared identical 16S rRNA gene sequences but their genomic fingerprint patterns differed, indicating the genetic distinctiveness of the two strains. A comparison of the 16S rRNA gene sequence of strain M1-83T with sequences of the type strains of the most closely related Paracoccus species showed highest sequence similarities to Paracoccus acridae (98.2â%) and Paracoccus aerius (98.1â%). 16S rRNA gene sequence similarities to all other Paracoccus species were below 97.6â%. The fatty acid profile of the two strains consisted mainly of the major fatty acids C18ââ1 ω7c and C180, which is typical for the genus Paracoccus. The polyamine patterns of strain M1-83T contained major amounts of putrescine and spermidine. The major quinone was ubiquinone Q-10. The diamino acid of the peptidoglycan was meso-diaminopimelic acid. The polar lipid profile was characterized by the major lipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and an unidentified glycolipid. DNA-DNA hybridization experiments between M1-83T and the type strains of P. acridae and P. aerius resulted in similarity values of 17â% (reciprocal, 60â%) and 23â% (reciprocal 30â%), respectively. DNA-DNA hybridization results together with the differentiating biochemical and chemotaxonomic properties showed that strain M1-83T represents a novel Paracoccusspecies, for which the name Paracoccus haematequi sp. nov. (type strain M1-83T=LMG 30633T=CIP 111624T=CCM 8857T), is proposed.
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MEDLINE
Assunto principal:
Paracoccus
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Filogenia
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Sangue
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Cavalos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article