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Conformation and Rheological Properties of Calf-Thymus DNA in Solution.
Bravo-Anaya, Lourdes Mónica; Rinaudo, Marguerite; Martínez, Félix Armando Soltero.
Afiliação
  • Bravo-Anaya LM; Grenoble Alpes University, Laboratoire Rhéologie et Procédés (LRP), F-38000, Grenoble 38000, France. monik_ayanami@hotmail.com.
  • Rinaudo M; Departamento de Ingeniería Química, Universidad de Guadalajara, Blvd. M. García Barragán, Guadalajara C.P. 44430, Jalisco, Mexico. monik_ayanami@hotmail.com.
  • Martínez FAS; Biomaterials applications, 6 rue Lesdiguières, Grenoble 38000, France. marguerite.rinaudo@sfr.fr.
Polymers (Basel) ; 8(2)2016 Feb 11.
Article em En | MEDLINE | ID: mdl-30979142
Studies of DNA molecule behavior in aqueous solutions performed through different approaches allow assessment of the solute-solvent interactions and examination of the strong influence of conformation on its physicochemical properties, in the presence of different ionic species and ionic concentrations. Firstly, the conformational behavior of calf-thymus DNA molecules in TE buffer solution is presented as a function of temperature. Secondly, their rheological behavior is discussed, as well as the evidence of the critical concentrations, i.e., the overlap and the entanglement concentrations (C* and Ce, respectively) from steady state flow and oscillatory dynamic shear experiments. The determination of the viscosity in the Newtonian plateau obtained from flow curves η ( ) allows estimation of the intrinsic viscosity and the specific viscosities at zero shear when C[η] < 40. At end, a generalized master curve is obtained from the variation of the specific viscosity as a function of the overlap parameter C[η]. The variation of the exponent s obtained from the power law η~ -s for both flow and dynamic results is discussed in terms of Graessley's analysis. In the semi-dilute regime with entanglements, a dynamic master curve is obtained as a function of DNA concentration (CDNA > 2.0 mg/mL) and temperature (10 °C < T < 40 °C).
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2016 Tipo de documento: Article