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Choline Uptake and Metabolism Modulate Macrophage IL-1ß and IL-18 Production.
Sanchez-Lopez, Elsa; Zhong, Zhenyu; Stubelius, Alexandra; Sweeney, Shannon R; Booshehri, Laela M; Antonucci, Laura; Liu-Bryan, Ru; Lodi, Alessia; Terkeltaub, Robert; Lacal, Juan Carlos; Murphy, Anne N; Hoffman, Hal M; Tiziani, Stefano; Guma, Monica; Karin, Michael.
Afiliação
  • Sanchez-Lopez E; Departments of Pharmacology and Pathology, University of California San Diego, La Jolla, CA, 92037, USA.
  • Zhong Z; Departments of Pharmacology and Pathology, University of California San Diego, La Jolla, CA, 92037, USA; Department of Immunology, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas TX 75390, USA.
  • Stubelius A; Division of Rheumatology, Allergy and Immunology, University of California San Diego, La Jolla, CA, 92037, USA.
  • Sweeney SR; Department of Nutritional Sciences, The University of Texas at Austin, Austin, TX, 78723-3092, USA.
  • Booshehri LM; Department of Pediatrics and Rady Children's Hospital, University of California San Diego, La Jolla, CA, 92037, USA.
  • Antonucci L; Departments of Pharmacology and Pathology, University of California San Diego, La Jolla, CA, 92037, USA.
  • Liu-Bryan R; Division of Rheumatology, Allergy and Immunology, University of California San Diego, La Jolla, CA, 92037, USA; VA San Diego Healthcare System, University of California San Diego, La Jolla, CA, 92037, USA.
  • Lodi A; Department of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX, 78723-3092, USA; Department of Nutritional Sciences, The University of Texas at Austin, Austin, TX, 78723-3092, USA.
  • Terkeltaub R; Division of Rheumatology, Allergy and Immunology, University of California San Diego, La Jolla, CA, 92037, USA; VA San Diego Healthcare System, University of California San Diego, La Jolla, CA, 92037, USA.
  • Lacal JC; Translational Oncology, Department of Oncology, Hospital Universitario Fuenlabrada, Instituto de Investigación Sanitaria IdiPAZ, Madrid, Spain.
  • Murphy AN; Departments of Pharmacology and Pathology, University of California San Diego, La Jolla, CA, 92037, USA.
  • Hoffman HM; Department of Pediatrics and Rady Children's Hospital, University of California San Diego, La Jolla, CA, 92037, USA.
  • Tiziani S; Department of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX, 78723-3092, USA; Department of Nutritional Sciences, The University of Texas at Austin, Austin, TX, 78723-3092, USA; Department of Pediatrics, Dell Medical School, The University of Texas
  • Guma M; Division of Rheumatology, Allergy and Immunology, University of California San Diego, La Jolla, CA, 92037, USA.
  • Karin M; Departments of Pharmacology and Pathology, University of California San Diego, La Jolla, CA, 92037, USA. Electronic address: karinoffice@ucsd.edu.
Cell Metab ; 29(6): 1350-1362.e7, 2019 06 04.
Article em En | MEDLINE | ID: mdl-30982734
ABSTRACT
Choline is a vitamin-like nutrient that is taken up via specific transporters and metabolized by choline kinase, which converts it to phosphocholine needed for de novo synthesis of phosphatidylcholine (PC), the main phospholipid of cellular membranes. We found that Toll-like receptor (TLR) activation enhances choline uptake by macrophages and microglia through induction of the choline transporter CTL1. Inhibition of CTL1 expression or choline phosphorylation attenuated NLRP3 inflammasome activation and IL-1ß and IL-18 production in stimulated macrophages. Mechanistically, reduced choline uptake altered mitochondrial lipid profile, attenuated mitochondrial ATP synthesis, and activated the energy sensor AMP-activated protein kinase (AMPK). By potentiating mitochondrial recruitment of DRP1, AMPK stimulates mitophagy, which contributes to termination of NLRP3 inflammasome activation. Correspondingly, choline kinase inhibitors ameliorated acute and chronic models of IL-1ß-dependent inflammation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Colina / Interleucina-18 / Interleucina-1beta / Macrófagos Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Colina / Interleucina-18 / Interleucina-1beta / Macrófagos Idioma: En Ano de publicação: 2019 Tipo de documento: Article