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A photocleavable surfactant for top-down proteomics.
Brown, Kyle A; Chen, Bifan; Guardado-Alvarez, Tania M; Lin, Ziqing; Hwang, Leekyoung; Ayaz-Guner, Serife; Jin, Song; Ge, Ying.
Afiliação
  • Brown KA; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
  • Chen B; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
  • Guardado-Alvarez TM; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
  • Lin Z; Department of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
  • Hwang L; Human Proteomics Program, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
  • Ayaz-Guner S; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
  • Jin S; Department of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
  • Ge Y; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
Nat Methods ; 16(5): 417-420, 2019 05.
Article em En | MEDLINE | ID: mdl-30988469
We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS-polyacrylamide gel electrophoresis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dodecilsulfato de Sódio / Tensoativos / Compostos Azo / Proteômica / Eletroforese em Gel de Poliacrilamida Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dodecilsulfato de Sódio / Tensoativos / Compostos Azo / Proteômica / Eletroforese em Gel de Poliacrilamida Idioma: En Ano de publicação: 2019 Tipo de documento: Article