BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing.
Genome Biol
; 20(1): 71, 2019 04 19.
Article
em En
| MEDLINE
| ID: mdl-30999927
ABSTRACT
Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3' cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR.
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Base de dados:
MEDLINE
Assunto principal:
Biblioteca Gênica
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Análise de Sequência de RNA
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Perfilação da Expressão Gênica
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article