Dihydroartemisinin represses esophageal cancer glycolysis by down-regulating pyruvate kinase M2.
Eur J Pharmacol
; 854: 232-239, 2019 Jul 05.
Article
em En
| MEDLINE
| ID: mdl-31004604
ABSTRACT
Esophageal cancer, especially esophageal squamous cell carcinoma (ESCC) threatens so many lives in China every year. Traditional treatment of ESCC has usually been disappointing. The development of novel therapy is worth investigation. We have previously demonstrated that dihydroartemisinin (DHA) has anticancer effect on esophageal cancer. However, the mechanism has not been completely known. In this present study, we explored the effect of DHA on cancer cell glycolysis, also known as Warburg effect. Pyruvate kinase M2 (PKM2) is a key regulatory factor of glycolysis, and our results showed that it is significantly overexpressed in patients with ESCC and ESCC cell lines. In DHA treatment cells, PKM2 was down-regulated and lactate product and glucose uptake were inhibited. Overexpression of PKM2 by lentiviral transfection abrogated the inhibition effect of DHA. These results suggested that DHA might repress esophageal cancer glycolysis partly by down-regulating PKM2 expression. We believe that DHA might be a prospective agent against esophageal cancer.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hormônios Tireóideos
/
Neoplasias Esofágicas
/
Proteínas de Transporte
/
Regulação para Baixo
/
Artemisininas
/
Glicólise
/
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article