Instantaneous isotropic volumetric imaging of fast biological processes.
Nat Methods
; 16(6): 497-500, 2019 06.
Article
em En
| MEDLINE
| ID: mdl-31036959
ABSTRACT
To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
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Análise de Célula Única
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Coração
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Microscopia
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article