Kinetics of infectious virus and viral RNA copy number in the blood of olive flounder infected with viral hemorrhagic septicemia virus (VHSV).

ABSTRACT

Viral hemorrhagic septicemia (VHS) is a cold-water disease caused by viral hemorrhagic septicemia virus (VHSV) at an optimal temperature of 9 °C-15 °C. VHSV isolation and detection have been accomplished by using a number of diagnostic methods such as cell culture and qRT-PCR. Spleen and kidney have been reported as the main target organs of VHSV-infection; however, how VHSV spreads throughout the fish body has not been clearly studied. The purpose of this study was 1) to investigate viral titer and viral RNA copy number in the blood of VHSV-infected olive flounder at 10 °C and 13 °C; 2) to compare VHSV titer and viral RNA copy numbers in blood from fish exposed to the virus by two different challenges. VHSV titer at 10 °C was higher than at 13 °C in blood samples of injection challenged group. Whereas, similar titer was observed at 10 °C and 13 °C in the blood samples of the immersion challenged group. At 10 °C, copy numbers of VHSV-N gene in blood of immersion challenged group increased slightly in comparison to injection challenged group. At 13 °C, similar patterns were observed between the injection and immersion challenged groups. Also, higher titer and copy number were observed in fish blood compared to tested organs from our previous study. Our results indicate that VHSV genome existed in fish blood at earlier time points after infection, and the blood may contribute to the spread of the virus in whole fish body. In addition, VHSV diagnosis by qRT-PCR from fish blood samples, not requiring sacrificing the host fish can be valuable to collect the kinetic information of viral infection.