A Bio-inspired Hypoxia Sensor using HIF1a-Oxygen-Dependent Degradation Domain.
Sci Rep
; 9(1): 7117, 2019 05 08.
Article
em En
| MEDLINE
| ID: mdl-31068630
ABSTRACT
Functional imaging has become an important tool in oncology because it not only provides information about the size and localization of the tumour, but also about the pathophysiological features of the tumoural cells. One of the characteristic features of some tumour types is that their fast growth leads to deficient intratumoral vascularization, which results in low oxygen availability. To overcome this lack of oxygen, tumoural cells activate the neoangiogenic program by upregulating the transcription factor HIF-1α. Herein we report a non-invasive in vitro detection method of hypoxia using designed fluorescent peptide probes based on the oxygen-dependent degradation domain of HIF-1α. The fluorescent probe retains the oxygen-sensing capability of HIF-1α, so that it is stabilized under hypoxia and readily degraded by the proteasome under normoxia, thus providing direct information of the cellular oxygen availability.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
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Neoplasias da Mama
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Técnicas Biossensoriais
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Hipóxia Celular
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Subunidade alfa do Fator 1 Induzível por Hipóxia
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Proteólise
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Domínios Proteicos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article