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Gold induces a pseudo-allergic reaction via MRGPRX2 both in vitro and in vivo.
Zeng, Yingnan; Wang, Jue; Zhang, Yongjing; Ge, Shuai; Wu, Yuanyuan; Fan, Ting; Wang, Nan.
Afiliação
  • Zeng Y; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
  • Wang J; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
  • Zhang Y; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
  • Ge S; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
  • Wu Y; Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China.
  • Fan T; Department of Pharmacy, Hong Hui Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710054, China.
  • Wang N; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: wangnan2014@xjtu.edu.cn.
Cell Immunol ; 341: 103923, 2019 07.
Article em En | MEDLINE | ID: mdl-31076078
The traditional mast cell (MC) degranulation pathway is mediated by crossing-linking of high-affinity IgE receptor (FcεRI), whereas a non-traditional, but analogous, pseudo-allergic way was recently reported to occur via Mas-Related G Protein-Coupled Receptor X2 (MRGPRX2). Severe contact hypersensitivity to metallic gold, typically considered non-sensitizing, has been reported. However, whether gold induces IgE-independent allergy remains unclear. Therefore, this study assessed the effects of gold chloride (CA) on MC activation and its relation to MRGPRX2. Our data show that CA acted on MRGPRX2 to increase cellular calcium levels and induced the release of inflammatory mediators in vitro. Compared to Mrgprb2-knockout (KO) mice, CA dose-dependently induced passive cutaneous anaphylaxis (PCA) in wild-type (WT) mice. Furthermore, peritoneal mast cells (MPMCs) were extracted from WT and Mrgprb2-KO mice and stimulated by CA, but only MPMCs from WT mice could be activated. Our results suggest that CA-induced pseudo-allergic responses are MRGPRX2 dependent.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Degranulação Celular / Receptores de Neuropeptídeos / Compostos de Ouro / Receptores Acoplados a Proteínas G / Dermatite de Contato / Mastócitos / Proteínas do Tecido Nervoso Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Degranulação Celular / Receptores de Neuropeptídeos / Compostos de Ouro / Receptores Acoplados a Proteínas G / Dermatite de Contato / Mastócitos / Proteínas do Tecido Nervoso Idioma: En Ano de publicação: 2019 Tipo de documento: Article