Isolation and enhancement of resveratrol production in Xylaria psidii by exploring the phenomenon of epigenetics: using DNA methyltransferases and histone deacetylase as epigenetic modifiers.

Resveratrol is an important stilbene which is having a high demand due to its therapeutic, cosmeceutical and nutraceutical activities. The current study mainly focuses on strategies to enhance the fungal potential to produce resveratrol via the activation of the cryptic biosynthetic pathway with their particular interest in the antioxidant application. The endophytic fungus Xylaria psidii was isolated from the surface sterilized leaf of Vitis vinifera. With the help of HPLC analysis it is found that resveratrol concentration was maximum and enhanced in case of treatment with 5 µm SAHA (52.32 µg/mL) and by 10 µm AZA (48.94 µg/mL) followed by 10 µm SAHA (41.10 µg/mL) and 5 µm AZA (37.72 µg/mL). After treatment with different concentration of epigenetic modifiers such as HDAC inhibitors (SAHA) and dMNTs (AZA) inhibitors, a significant increase in antioxidant potential was obtained. In the case of DPPH increase in scavenging potential was found as compared to wild strain. Treatment with 5 µm SAHA and by 10 µm AZA was showing strong antioxidant potential among all the epigenetic variants as compared to wild strain. In the case of TEAC also the same trend as in the case of DPPH was obtained.