Development and characterization of ten polymorphic microsatellites of the Western Ghats frog Indirana semipalmata and cross amplification in related species.

ABSTRACT

The genus Indirana Laurent, 1986 is composed of 14 species which are endemic to the Western Ghats of India. We isolated and evaluated ten polymorphic microsatellite markers in 32 individuals of Indirana semipalmata. The cross amplification test was successful in two Indirana (I. brachytarsus and I. yadera) and one Walkerana (W. leptodactyla, previously known as I. leptodactyla) species endemic to the Western Ghats. The identified markers will be useful for further studies on the conservation genetics of endemic frog species of Western Ghats.