miR4905p regulates the proliferation, migration, invasion and epithelialmesenchymal transition of pharyngolaryngeal cancer cells by targeting mitogenactivated protein kinase kinasekinase 9.
Int J Mol Med
; 44(1): 240-252, 2019 Jul.
Article
em En
| MEDLINE
| ID: mdl-31115491
ABSTRACT
MicroRNA (miRNA/miR) has been identified to be a promising tool in treating pharyngolaryngeal cancer. The present study aimed to investigate the role of miR4905p in the regulation of proliferation, migration, invasion and epithelialmesenchymal transition (EMT) of pharyngolaryngeal cancer cells. The data of miR4905p expression levels of 45 cases were obtained from the People's Hospital of Xinjiang Uygur Autonomous Region, and the prediction of the target of miR4905p was conducted by bioinformatics and verified using a luciferase assay. Cell viability was determined by cell counting kit8. Migration and invasion rates were measured by wound healing test and Transwell apparatus, respectively. Colony formation rate was measured by plate colony formation assay. mRNA and protein levels were determined by quantitative polymerase chain reaction and western blotting, respectively. miR4905p expression was significantly depressed in primary pharyngolaryngeal cancer tissues and cell lines, leading to an unfavorable prognosis. Evidently, miR4905p overexpression decreased the cell viabilities of BICR 18 and FaDu cells. Mechanically, miR4905p could target mitogenactivated protein kinase kinasekinase 9 (MAP3K9). The overexpression of MAP3K9 could promote cell viability, migration and invasion rates, EMT process and ability of cloning, miR4905p could target MAP3K9 and further modulate the proliferation, migration, invasion and EMT of pharyngolaryngeal cancer cells. The results of the present study provide a novel entry point to the treatment of pharyngolaryngeal cancer.
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Base de dados:
MEDLINE
Assunto principal:
RNA Neoplásico
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Neoplasias Faríngeas
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Movimento Celular
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Neoplasias Laríngeas
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MAP Quinase Quinase Quinases
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MicroRNAs
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Proliferação de Células
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Transição Epitelial-Mesenquimal
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Proteínas de Neoplasias
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article