Human high-density lipoprotein microtranscriptome is unique and suggests an extended role in lipid metabolism.
Epigenomics
; 11(8): 917-934, 2019 06.
Article
em En
| MEDLINE
| ID: mdl-31144512
ABSTRACT
Aim:
To comprehensively characterize the high-density lipoproteins (HDLs) microtranscriptome and to assess whether it is distinct from that of plasma and different between women and men.Methods:
RNA was extracted from ultracentrifugation-purified HDLs and plasma from 17 healthy women and men couples, and libraries were sequenced on a HiSeq2500 platform.Results:
On average, 310 ± 64 and 355 ± 31 miRNAs were detected (≥1 read per million) in HDLs and plasma, respectively. A total of 62 and 134 miRNAs were over-represented (e.g., miR-150-5p; fold change = 7.52; padj = 5.41 × 10-111) and under-represented (e.g., miR-22-3p; fold change = -5.28; padj = 2.11 × 10-154) in HDLs compared with plasma. These miRNAs were enriched in lipid metabolism and cellular processes-related pathways.Conclusion:
HDLs exhibit a sex-independent miRNA profile distinct from that of plasma. These miRNAs may contribute to the HDLs' physiology.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
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Metabolismo dos Lipídeos
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Transcriptoma
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Lipoproteínas HDL
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article