Label-free multi-parametric imaging of single cells: dual picosecond optoacoustic microscopy.
J Biophotonics
; 12(8): e201900045, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-31144774
ABSTRACT
Advances in microscopy with new visualization possibilities often bring dramatic progress to our understanding of the intriguing cellular machinery. Picosecond optoacoustic micro-spectroscopy is an optical technique based on ultrafast pump-probe generation and detection of hypersound on time durations of picoseconds and length scales of nanometers. It is experiencing a renaissance as a versatile imaging tool for cell biology research after a plethora of applications in solid-state physics. In this emerging context, this work reports on a dual-probe architecture to carry out real-time parallel detection of the hypersound propagation inside a cell that is cultured on a metallic substrate, and of the hypersound reflection at the metal/cell adhesion interface. Using this optoacoustic modality, several biophysical properties of the cell can be measured in a noncontact and label-free manner. Its abilities are demonstrated with the multiple imaging of a mitotic macrophage-like cell in a single run experiment.
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Base de dados:
MEDLINE
Assunto principal:
Análise de Célula Única
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Técnicas Fotoacústicas
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Microscopia
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article