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Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system.
Ingle, Patrick; Groothuis, Daphne; Rowe, Peter; Huang, He; Cockayne, Alan; Kuehne, Sarah A; Jiang, Weihong; Gu, Yang; Humphreys, Christopher M; Minton, Nigel P.
Afiliação
  • Ingle P; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK.
  • Groothuis D; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK.
  • Rowe P; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK.
  • Huang H; Deep Branch Biotechnology, NG8 1AA, Nottingham, United Kingdom.
  • Cockayne A; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
  • Kuehne SA; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK.
  • Jiang W; Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, The University of Nottingham, Nottingham, NG7 2RD, UK.
  • Gu Y; School of Dentistry, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, B5 7SA, UK.
  • Humphreys CM; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
  • Minton NP; Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
Sci Rep ; 9(1): 8123, 2019 05 31.
Article em En | MEDLINE | ID: mdl-31148548
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Eritromicina / Clostridioides difficile / Genoma Bacteriano / Sistemas CRISPR-Cas / Edição de Genes Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Eritromicina / Clostridioides difficile / Genoma Bacteriano / Sistemas CRISPR-Cas / Edição de Genes Idioma: En Ano de publicação: 2019 Tipo de documento: Article