Rational design of a function-based selection method for genetically encoding acylated lysine derivatives.
Org Biomol Chem
; 17(25): 6127-6130, 2019 06 26.
Article
em En
| MEDLINE
| ID: mdl-31172146
ABSTRACT
A foundation of genetic code expansion is the evolution of orthogonal aminoacyl-tRNA synthetase to recognize a non-canonical amino acid, which typically involves read-through of amber stop codon in the genes used in selection. The process is time-consuming, labor-intensive, and susceptible to certain bias. As a complementation, herein, we rationally designed a function-based method to directed evolution of aminoacyl-tRNA synthetase for acylated lysine derivatives.
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MEDLINE
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En
Ano de publicação:
2019
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Article