Zoledronic acid inhibits osteoclast differentiation and function through the regulation of NF-κB and JNK signalling pathways.

It is well known that extensive osteoclast formation plays a key role in osteoporosis in post­menopausal women and the elderly. The suppression of extensive osteoclastogenesis and bone resorption may be an effective preventive strategy for osteoporosis. Zoledronic acid (ZOL) has been indicated to play an essential role in regulating bone mineral density and has already been used in large clinical trials. However, the effects of ZOL on osteoclastogenesis remain to be fully elucidated. Therefore, the present study aimed to determine the effects of ZOL on osteoclastogenesis, and to explore the corresponding signalling pathways. By using a cell viability assay, as well as in vitro osteoclastogenesis, immunofluorescence and resorption pit assays, we demonstrated that ZOL (0.1­5 µM) suppressed receptor activator of nuclear factor­κB ligand (RANKL)­induced osteoclast differentiation and bone resorptive activity. Furthermore, western blot analysis and reverse transcription­quantitative PCR indicated that ZOL inhibited the RANKL­induced activation of NF­κB and the phosphorylation of JNK in RAW264.7 cells, and subsequently decreased the expression of osteoclastogenesis­associated genes, including calcitonin receptor, tartrate­resistant acid phosphatase and dendritic cell­specific transmembrane protein. ZOL inhibited osteoclast formation and resorption in vitro by specifically suppressing NF­κB and JNK signalling. On the whole, the findings of this study indicate that ZOL may serve as a potential agent for the treatment of osteoclast­associated diseases, including osteoporosis.