Unravelling the Single-Stranded DNA Virome of the New Zealand Blackfly.
Viruses
; 11(6)2019 06 08.
Article
em En
| MEDLINE
| ID: mdl-31181730
ABSTRACT
Over the last decade, arthropods have been shown to harbour a rich diversity of viruses. Through viral metagenomics a large diversity of single-stranded (ss) DNA viruses have been identified. Here we examine the ssDNA virome of the hematophagous New Zealand blackfly using viral metagenomics. Our investigation reveals a plethora of novel ssDNA viral genomes, some of which cluster in the viral families Genomoviridae (n = 9), Circoviridae (n = 1), and Microviridae (n = 108), others in putative families that, at present, remain unclassified (n = 20) and one DNA molecule that only encodes a replication associated protein. Among these novel viruses, two putative multi-component virus genomes were recovered, and these are most closely related to a Tongan flying fox faeces-associated multi-component virus. Given that the only other known multi-component circular replication-associated (Rep) protein encoding single-stranded (CRESS) DNA viruses infecting plants are in the families Geminiviridae (members of the genus Begomovirus) and Nanoviridae, it appears these are likely a new multi-component virus group which may be associated with animals. This study reiterates the diversity of ssDNA viruses in nature and in particular with the New Zealand blackflies.
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Base de dados:
MEDLINE
Assunto principal:
Simuliidae
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DNA de Cadeia Simples
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Genoma Viral
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Vírus de DNA
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article