Cu pre-exposure alters antioxidant defense and energy metabolism in large yellow croaker Larimichthys crocea in response to severe hypoxia.
Sci Total Environ
; 687: 702-711, 2019 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-31220723
ABSTRACT
The aim of the present study was to evaluate the effects of Cu pre-exposure on antioxidant defense and energy metabolism in the liver of the large yellow croaker exposed to severe hypoxia. Fish were pre-acclimated to 0 and 30⯵g Cu L-1 for 96â¯h, and subsequently exposed to 7.0 and 1.5â¯mg DO L-1 for another 24â¯h. Hypoxic stress alone increased reactive oxygen species and hepatic vacuoles. When compared to hypoxic stress alone, hypoxic stress plus Cu pre-exposure increased mortality and ROS production, and worsened histological structure by inhibiting antioxidant defense and aerobic metabolism, and enhancing anaerobic metabolism, suggesting Cu pre-acclimation aggravated hypoxia-induced oxidative damage. NFE2-related nuclear factor 2 and hypoxia-inducible factor-1α might participate in the transcriptional regulation of genes related to antioxidant response and energy metabolism, respectively. In conclusion, Cu pre-acclimation had a synergistic effect on antioxidant response and energy metabolism in fish under severe hypoxia, which contributes to understanding the molecular mechanisms underlying negative effects of Cu pre-acclimation against hypoxic damage in fish.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
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Cobre
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Metabolismo Energético
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Antioxidantes
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article