Isolation of canine adipose-derived mesenchymal stem cells from falciform tissue obtained via laparoscopic morcellation: A pilot study.
Vet Surg
; 49 Suppl 1: O28-O37, 2020 Jun.
Article
em En
| MEDLINE
| ID: mdl-31222769
ABSTRACT
OBJECTIVE:
To evaluate the feasibility of stem cell isolation from falciform fat harvested via laparoscopic morcellation. STUDYDESIGN:
Pilot study. ANIMALS Eleven client-owned dogs.METHODS:
Falciform was harvested traditionally via laparotomy and laparoscopically via tissue morcellation. Harvested tissue was processed with a commercially available adipose tissue dissociation kit to obtain a stromal vascular fraction (SVF). Cells were subsequently labeled for CD90, CD45, and CD44 cell surface antigens by using magnetic-activated cell sorting (MACS) and fluorescence-activated cell sorting flow cytometry. CD90+ cells were quantitated, and their viability was assessed with a hemocytometer and a trypan blue exclusion test of cell viability.RESULTS:
No perioperative complications occurred in dogs undergoing laparoscopic morcellation. Laparoscopically and traditionally harvested samples yielded an average of 0.39 (±0.1) × 106 and 0.33 (±0.1) × 106 CD90+ cells, respectively, per 10 million SVF cells. CD90+ cell viability after MACS was 89% (±11%) for morcellated and 86% (±7%) for traditionally harvested samples. Neither CD90+ cell quantity nor viability was different between samples obtained via traditional laparotomy vs laparoscopic morcellation (P = .38 and P = .63, respectively). Populations of CD90+ cells isolated with each harvest technique had similar CD44 and CD45 expression profiles.CONCLUSION:
Viable populations of CD90+ cells with similar CD44/CD45 expression profiles were isolated from laparoscopically morcellated and traditionally harvested falciform tissue. No appreciable morbidity was associated with laparoscopic falciform morcellation. CLINICALSIGNIFICANCE:
Laparoscopic morcellation is a safe and effective minimally invasive approach to falciform tissue harvest for adipose-derived mesenchymal stem cell isolation.
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Base de dados:
MEDLINE
Assunto principal:
Tecido Adiposo
/
Laparoscopia
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Coleta de Tecidos e Órgãos
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Cães
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Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article