Protective effects of bovine serum albumin on blueberry anthocyanins under illumination conditions and their mechanism analysis.
Food Res Int
; 122: 487-495, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-31229104
ABSTRACT
This study investigates the effects of bovine serum albumin (BSA) on blueberry anthocyanins and their interaction. Findings showed that BSA could protect blueberry anthocyanins against degradation and retain their antioxidant activity at an ideal concentration of 0.15â¯mg/mL under three deteriorating treatments illumination, vitamin Câ¯+â¯illumination, and sucrose + illumination. The fluorescence and UV absorption spectra showed that malvidin-3-o-galactoside (M3G), the major monomer in blueberry anthocyanins, led to a static quenching of BSA and the binding site of M3G to BSA was approximately one. Further, the interaction was a spontaneous process with electrostatic interactions being the main force. CD spectra and synchronous fluorescence spectra presented alterations in the secondary structure and microenvironment of Trp and Tyr residues of BSA, respectively, upon interaction with M3G. Finally, molecular docking analysis showed that M3G mainly bound the II and III domains of BSA by hydrogen bonds and electrostatic interaction. In conclusion, our study highlights the protective effects of BSA on the stability and anti-oxidant activity of blueberry anthocyanins and their interaction analysis.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Soroalbumina Bovina
/
Mirtilos Azuis (Planta)
/
Luz
/
Antocianinas
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article