A click chemistry assay to identify natural product ligands for pre-microRNAs.
Methods Enzymol
; 623: 85-99, 2019.
Article
em En
| MEDLINE
| ID: mdl-31239059
ABSTRACT
Despite the great diversity of structure and function and relevance to human health, RNA remains an underexploited area of drug discovery. A major bottleneck toward this goal has been the identification of probes and drug leads that are specific for select RNAs and methods that will facilitate such discovery efforts. Our laboratory has recently developed an innovative approach for assaying RNA-small molecule interactions, catalytic enzyme-linked click chemistry assay or cat-ELCCA, which is a functional assay that takes advantage of the power of catalytic signal amplification combined with the selectivity and bioorthogonality of click chemistry. Importantly, through application of this platform assay technology to the challenging problem of identifying selective inhibitors of pre-microRNA maturation, we identified natural products as a potential source of such compounds. Herein we describe this methodology in addition to the downstream pipeline toward the discovery of natural product ligands for pre-microRNAs. Through cat-ELCCA, our goal is to discover novel ligands to facilitate our investigation of RNA recognition by small molecules.
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Base de dados:
MEDLINE
Assunto principal:
Produtos Biológicos
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MicroRNAs
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Bibliotecas de Moléculas Pequenas
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Descoberta de Drogas
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Química Click
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article