A split fluorescent reporter with rapid and reversible complementation.
Nat Commun
; 10(1): 2822, 2019 06 27.
Article
em En
| MEDLINE
| ID: mdl-31249300
ABSTRACT
Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Mapeamento de Interação de Proteínas
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article