Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells.
Methods Mol Biol
; 1998: 63-72, 2019.
Article
em En
| MEDLINE
| ID: mdl-31250294
ABSTRACT
Proximity ligation assay (PLA) is a newly developed technique that outperforms the traditional immunoassays for visualizing the in situ endogenous protein-protein interactions and localizations and the activation of proteins in cell culture systems as well as in tissue sections. PLA, when combined with cellular marker staining, becomes a powerful approach to identify differential interaction of the proteins of endosomal sorting complex required for transport (ESCRT) at distinct stages of virus infection. In this chapter, we describe a PLA protocol to study the localization and interaction between the ESCRT protein TSG101 and endosomal markers in early stages of viral endocytosis in in vitro infected cells.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Endossomos
/
Fatores de Transcrição
/
Bioensaio
/
Herpesvirus Humano 8
/
Mapeamento de Interação de Proteínas
/
Proteínas de Ligação a DNA
/
Complexos Endossomais de Distribuição Requeridos para Transporte
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article