Quantification and assessment of detection capability in imaging mass spectrometry using a revised mimetic tissue model.
Bioanalysis
; 11(11): 1099-1116, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-31251106
ABSTRACT
Aim:
A revised method of preparing the mimetic tissue model for quantitative imaging mass spectrometry (IMS) is evaluated. Concepts of assessing detection capability are adapted from other imaging or mass spectrometry (MS)-based technologies to improve upon the reliability of IMS quantification. Materials &methods:
The mimetic tissue model is prepared by serially freezing spiked-tissue homogenates into a cylindrical mold to create a plug of tissue with a stepped concentration gradient of matrix-matched standards. Weighted least squares (WLS) linear regression is applied due to the heteroscedastisity (change in variance with intensity) of most MS data. Results &conclusions:
Imaging poses several caveats for quantification which are unique compared with other MS-based methods. Aspects of the design, construction, application, and evaluation of the matrix-matched standard curve for the mimetic tissue model are discussed. In addition, the criticality of the ion distribution in the design of a purposeful liquid chromatography coupled to mass spectrometry (LC-MS) validation is reviewed.Palavras-chave
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MEDLINE
Assunto principal:
Pele
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Clorpropamida
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Clozapina
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Fígado
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Modelos Biológicos
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Nucleosídeos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article