TLR4/NF-κB signaling pathway gene single nucleotide polymorphisms alter gene expression levels and affect ARDS occurrence and prognosis outcomes.
Medicine (Baltimore)
; 98(26): e16029, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-31261506
ABSTRACT
BACKGROUND:
To study the occurrence and prognosis of acute respiratory distress syndrome (ARDS) using single nucleotide polymorphisms (SNPs) of TNF-α rs1800629, IL-6 rs1800796, and MyD88 rs7744 loci in the TLR4/NF-κB pathway.METHODS:
Genotypes were analyzed for TNF-α rs1800629, IL-6 rs1800796, and MyD88 rs7744 loci. Plasma TNF-α and IL-6 levels and MyD88 mRNA expression in peripheral blood mononuclear cells (PBMCs) of 300 ARDS patients and 300 non-ARDS patients (control group) were examined. The patients were followed up for 60 days, and the prognosis outcome was recorded.RESULTS:
The TNF-α rs1800629 locus A allele and the IL-6 rs1800796 locus G allele were found to be risk factors for ARDS (adjusted ORâ=â1.452, 95% CI 1.211-1.689, Pâ<â.001 and adjusted ORâ=â1.205, 95% CI 1.058-1.358, Pâ=â.005, respectively). The G allele at MyD88 rs7744 locus was a protective factor against ARDS (adjusted ORâ=â0.748, 95% CI 0.631-0.876, Pâ<â.001). Compared with the other groups, homozygotes for TNF-α rs1800629, IL-6 rs1800796, and MyD88 rs7744 loci had higher expression levels, of which homozygotes for TNF-α rs1800629 and IL-6 rs1800796 loci had lower 60-day survival rates, while MyD88 rs7744 locus homozygotes had a higher 60-day survival rate.CONCLUSION:
The effect of TNF-α rs1800629, IL-6 rs1800796, and MyD88 rs7744 SNPs on gene expression level is a likely cause of ARDS occurrence and poor prognosis.
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Base de dados:
MEDLINE
Assunto principal:
Síndrome do Desconforto Respiratório
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NF-kappa B
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Fator de Necrose Tumoral alfa
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Polimorfismo de Nucleotídeo Único
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Receptor 4 Toll-Like
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article