Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy.
Int J Mol Sci
; 20(13)2019 Jul 03.
Article
em En
| MEDLINE
| ID: mdl-31277289
ABSTRACT
Atomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 µM) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized to determine simultaneous variations of Young's modulus, the maximum adhesion force, and tether formation, thereby determining cell motility and adhesiveness. Effects of treatment were measured at several time-points (0-6 h, 24 h, and 48 h); longer exposures resulted in cell death. Our results demonstrated that AFM can be efficiently used as a diagnostic tool to monitor irreversible morpho/nano-mechanical changes in cancer cells during the early steps of drug treatment.
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MEDLINE
Assunto principal:
Neoplasias da Mama
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Adesão Celular
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Movimento Celular
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Microscopia de Força Atômica
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Módulo de Elasticidade
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Resveratrol
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article