Direct Detection of Circulating Tumor Cells in Whole Blood Using Time-Resolved Luminescent Lanthanide Nanoprobes.
Angew Chem Int Ed Engl
; 58(35): 12195-12199, 2019 08 26.
Article
em En
| MEDLINE
| ID: mdl-31286625
ABSTRACT
The detection of circulating tumor cells (CTCs) is crucial to early cancer diagnosis and the evaluation of cancer metastasis. However, it remains challenging due to the scarcity of CTCs in the blood. Herein, we report an ultrasensitive platform for the direct detection of CTCs using luminescent lanthanide nanoprobes. These were designed to recognize the epithelial cell adhesion molecules on cancer cells, allowing signal amplification through dissolution-enhanced time-resolved photoluminescence (TRPL) and the elimination of short-lived autofluorescence interference. This enabled the direct detection of blood breast-cancer cells with a limit of detection down to 1 cell/well of a 96-well plate. Moreover, blood CTCs (≥10â
cells mL-1 ) can be detected in cancer patients with a detection rate of 93.9 % (14/15 patients). We envision that this ultrasensitive detection platform with excellent practicality may provide an effective strategy for early cancer diagnosis and prognosis evaluation.
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MEDLINE
Assunto principal:
Neoplasias da Mama
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Elementos da Série dos Lantanídeos
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Nanopartículas Metálicas
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Células Neoplásicas Circulantes
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article