Complete Biotransformation of Protopanaxadiol-Type Ginsenosides into 20-O-ß-Glucopyranosyl-20(S)-protopanaxadiol by Permeabilized Recombinant Escherichia coli Cells Coexpressing ß-Glucosidase and Chaperone Genes.
J Agric Food Chem
; 67(30): 8393-8401, 2019 Jul 31.
Article
em En
| MEDLINE
| ID: mdl-31291721
ABSTRACT
The ginsenoside 20-O-ß-glucopyranosyl-20(S)-protopanaxadiol or compound K is an essential ingredient in functional food, cosmetics, and traditional medicines. However, no study has reported the complete conversion of all protopanaxadiol (PPD)-type ginsenosides from ginseng extract into compound K using whole-cell conversion. To increase the production of compound K from ginseng extract using whole recombinant cells, the ß-glucosidase enzyme from Caldicellulosiruptor bescii was coexpressed with a chaperone expression system (pGro7), and the cells expressing the coexpression system were permeabilized with ethylenediaminetetraacetic acid. The permeabilized cells carrying the chaperone coexpression system showed a 2.6-fold increase in productivity and yield as compared with nontreated cells, and completely converted all PPD-type ginsenosides from ginseng root extract into compound K with the highest productivity among the results reported so far. Our results will contribute to the industrial biological production of compound K.
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Base de dados:
MEDLINE
Assunto principal:
Sapogeninas
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Proteínas de Bactérias
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Beta-Glucosidase
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Chaperonas Moleculares
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Ginsenosídeos
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Escherichia coli
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Firmicutes
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article