Effects of leptin on the follicular development and mitochondrial activity of ovine isolated early antral follicles cultured in vitro.
Theriogenology
; 138: 77-83, 2019 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-31302434
ABSTRACT
This study evaluated the effect of leptin on the in vitro culture of isolated sheep early antral follicles. Early antral follicles (300-450⯵m) were isolated and cultured for 12 days in tissue culture medium 199 (TCM 199) supplemented with glutamine, hypoxanthine, transferrin, insulin, selenium, ascorbic acid, bovine serum albumin (BSA) and recombinant follicle stimulating hormone (rFSH) (TCM 199+ control medium) or TCM 199+ supplemented with 2 or 10â¯ng/mL leptin. After culture, oocytes were subjected to in vitro maturation (IVM). The parameters analyzed were morphology, extrusion rate, follicular diameter, growth and fully-grown oocytes (oocytes ≥110⯵m) rates. After IVM, reactive oxygen species (ROS) levels, mitochondrial activity, meiotic stages and meiotic resumption rates were also analyzed. After 12 days of culture, the concentration of 2â¯ng/mL of leptin showed a higher percentage of morphologically normal follicles, fully-grown oocytes (≥110⯵m), active mitochondria and meiotic resumption compared to the control medium (TCM 199+; Pâ¯<â¯0.05) but did not differ when compared to leptin concentration of 10â¯ng/mL (Pâ¯>â¯0.05). After culturing, no significant differences existed among treatments in terms of the follicle diameter and ROS levels. In conclusion, the addition of 2â¯ng/mL leptin to the base culture medium is capable of improving follicular survival, oocyte growth, mitochondrial activity and meiotic resumption after the in vitro culture of isolated sheep early antral follicles.
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MEDLINE
Assunto principal:
Leptina
/
Folículo Ovariano
/
Mitocôndrias
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article