Construction of a novel Escherichia coli expression system: relocation of lpxA from chromosome to a constitutive expression vector.

Zhao, Lei; Hu, Xiaoqing; Li, Ye; Wang, Zhen; Wang, Xiaoyuan

Zhao, Lei; Hu, Xiaoqing; Li, Ye; Wang, Zhen; Wang, Xiaoyuan.

Afiliação

Zhao L; State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
Hu X; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China.
Li Y; State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
Wang Z; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China.
Wang X; State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.

Appl Microbiol Biotechnol ; 103(17): 7177-7189, 2019 Sep.

Article em En | MEDLINE | ID: mdl-31317228

Assuntos

Aciltransferases/genética; Cromossomos Bacterianos/genética; Escherichia coli/genética; Vetores Genéticos/genética; Deleção de Genes; Expressão Gênica; Genes Essenciais/genética; Teste de Complementação Genética; Plasmídeos/genética; Treonina/biossíntese

Palavras-chave

Cas9; Escherichia coli; Expression system; L-threonine production; lpxA; lpxA-carrying vector

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Aciltransferases / Cromossomos Bacterianos / Escherichia coli / Vetores Genéticos Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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