Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.
Carbohydr Polym
; 222: 114962, 2019 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-31320077
ABSTRACT
The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing. Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce. In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity. A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides. These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (ß1â3, ß1â4)-glucans, (α1â3)-, (α1â4)-mannans, and anionic sulphated heterorhamnans. The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes. Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Linfócitos B
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Estramenópilas
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Microalgas
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article