Long noncoding RNA plasmacytoma variant translocation 1 gene promotes the development of cervical cancer via the NFκB pathway.
Mol Med Rep
; 20(3): 2433-2440, 2019 Sep.
Article
em En
| MEDLINE
| ID: mdl-31322217
ABSTRACT
The long noncoding RNA plasmacytoma variant translocation 1 gene (LncRNA PVT1) has an important role in tumor occurrence and development, yet the role and underlying molecular mechanisms of this RNA in cervical cancer have not yet been elucidated. In the present study, three cervical cancer cell lines (HeLa, Ca Ski and SiHa) were used to verify how LncRNA PVT1 mediates cervical cancer development, and the H8 cell line was used as a control. A LncRNA PVT1 overexpression vector or small interfering RNAs targeting LncRNA PVT1 were transfected into cervical cancer cells to generate LncRNA PVT1 overexpression and silencing in these cells. LncRNA PVT1 overexpression accelerated the growth of cervical cancer cells by advancing the cell cycle and inhibiting cellular apoptosis; increases in Cyclin D1 (CCND1) mRNA and activated Bcl2 protein expression levels also supported this finding. Furthermore, NFκB activation and expression was increased by LncRNA PVT1 overexpression. In addition, NFκB activation or inhibition induced changes in cell viability, accompanied by changes in CCND1 and Bcl2 expression. Increases or decreases in microRNA16 (miR16) expression (using miR mimics and inhibitors) also corresponded to changes in LncRNA PVT1 expression, in vitro. miR16 mimics and inhibitor had opposite effects to those of NFκB activity, and miR16 was demonstrated to directly interact with the NFκB gene as measured using the dualluciferase assay. In summary, LncRNA PVT1 inhibits the effect of miR16, promoting the cell cycle and inhibiting cellular apoptosis of cervical cancer cells, potentially via the NFκB pathway. The data from the present study will contribute to the current knowledge surrounding the theoretical basis of cervical cancer and provide a new perspective for the treatment of cervical cancer.
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Base de dados:
MEDLINE
Assunto principal:
Regulação Neoplásica da Expressão Gênica
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Neoplasias do Colo do Útero
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NF-kappa B
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RNA Longo não Codificante
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article