Three new steroidal components from the roots of reineckia carnea.

ABSTRACT

A new coprostanol glycoside, 26-trihydroxy-16, 22-dioxo-3ß-[(α-L-rhamnopyranosyl)oxy]-5ß-cholestan-1ß-yl O-α-L-rhamnopyranosyl-(1→2)-ß-D-fucopyranoside (1) (25S)-5ß-spirostan-1ß,2ß,3ß, 4ß,5ß, 6ß-hexol (2), a new C-22 steroidal lactone saponin, (20 R)-16ß-trihydroxy-3ß-[(α-L-rhamnopyranosyl)oxy]-1ß-[(O-α-L-rhamnopyranosyl-(1→2)-ß-D-xylopyranosyl)oxy]-5ß-pregnane-20-oic acid γ-lactone (3) along with two known pregnane glycosides (4 and 5) were obtained from the roots of Reineckia carnea. Their structures were determined by 1 D, 2 D NMR, IR and MS data analysis. In addition, the cytotoxic activities in HT-29, HCT116, H1299 and A549 tumor cells of the isolated compounds (1 - 5) were determined by the MTT method. The results showed that only compound 1 exhibited weak effect against these cells with IC50 values ranging from 63.36 µM to 105.01 µM.