Novel neuroprotective hydroquinones with a vinyl alkyne from the fungus, Pestalotiopsis microspora.
J Antibiot (Tokyo)
; 72(11): 793-799, 2019 11.
Article
em En
| MEDLINE
| ID: mdl-31341274
ABSTRACT
New hydroquinone derivatives bearing a vinyl alkyne, pestalotioquinols A and B, were isolated from a fungal culture broth of Pestalotiopsis microspora. The structures of these novel compounds were determined by interpretation of spectroscopic data (1D/2D NMR, MS, and IR), and the absolute configuration of the stereogenic center of pestalotioquinol A was assigned using the modified Mosher's method. Nerve growth factor-differentiated neuronal PC12 cells were pretreated with pestalotioquinols A and B and removed from the medium, and then treated with a generator of peroxynitrite (ONOO-), a reactive nitrogen species, to induce cell death. The cytotoxicity of the treated cells was assessed by measuring lactate dehydrogenase leakage. As a result, 1-3 µM pretreatment of pestalotioquinols A and B rescued neuronal PC12 cells from peroxynitrite-induced cytotoxicity and the protective activity was sustained after removing each compound from the medium. These results demonstrate that pestalotioquinol derivatives are a new class of hydroquinones possessing a vinyl alkyne and exhibiting relatively high neuroprotective effects.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ascomicetos
/
Hidroquinonas
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article