Interleukin-1ß promotes interleulin-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts.
PLoS One
; 14(7): e0220262, 2019.
Article
em En
| MEDLINE
| ID: mdl-31344106
ABSTRACT
Interleukin-6 (IL-6) is a pleiotropic cytokine involved in the regulation of the immune response and inflammation. In this study, we investigated effect of the proinflammatory cytokine interleukin-1ß (IL-1ß) on IL-6 expression in canine dermal fibroblasts. IL-1ß induced IL-6 mRNA expression and protein release in a time- and dose-dependent manner. When cells were treated with inhibitors of mitogen-activated protein kinases (MAPKs), the extracellular signal-regulated kinase (ERK) inhibitor FR180240 inhibited IL-1ß-induced IL-6 mRNA expression, but not SP600125 or SKF86002, which are c-Jun N-terminal kinase (JNK) and p38 MAPK inhibitors, respectively. In cells treated with U0126, an inhibitor of MAPK/ERK kinase (MEK), which activates ERK, IL-1ß-induced IL-6 mRNA expression was also inhibited. IL-1ß stimulated ERK1/2 phosphorylation. In cells transfected with ERK1 and ERK2 isoform siRNAs, IL-1ß-induced IL-6 mRNA expression was reduced. These observations suggest that IL-1ß induces IL-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Interleucina-6
/
Derme
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Interleucina-1beta
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Fibroblastos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article