Major Impact of Sampling Methodology on Gene Expression in Estrogen Receptor-Positive Breast Cancer.
JNCI Cancer Spectr
; 2(2): pky005, 2018 Apr.
Article
em En
| MEDLINE
| ID: mdl-31360844
ABSTRACT
To investigate the impact of sampling methodology on gene expression data from primary estrogen receptor-positive (ER+) breast cancer biopsies, global gene expression was measured in core-cut biopsies at baseline and surgery from patients randomly assigned to receive either two weeks of presurgical aromatase inhibitor (AI; n = 157) or no presurgical treatment (n = 56). Those genes most markedly altered in the AI group (eg, FOS, DUSP1, RGS1, FOSB) were similarly altered in the no treatment group; some widely investigated genes that were apparently unaffected in the AI group (eg, MYC) were counter-altered in the control group, masking actual AI-dependent changes. In the absence of a control group, these artefactual changes would likely lead to the most affected genes being the erroneous focus of research. The findings are likely relevant to all archival collections of ER+ breast cancer.
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MEDLINE
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En
Ano de publicação:
2018
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Article