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Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides.
Guo, Boyang; Itami, Jun; Oikawa, Kazusato; Motoda, Yoko; Kigawa, Takanori; Numata, Keiji.
Afiliação
  • Guo B; Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan.
  • Itami J; Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan.
  • Oikawa K; Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan.
  • Motoda Y; Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan.
  • Kigawa T; Laboratory for Cellular Structural Biology, RIKEN Center for Biosystems Dynamics Research, Yokohama, Kanagawa, Japan.
  • Numata K; Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan.
PLoS One ; 14(7): e0214033, 2019.
Article em En | MEDLINE | ID: mdl-31361745
Direct protein delivery into intact plants remains a challenge for the agricultural and plant science fields. Cell-penetrating peptide (CPP)-mediated protein delivery requires the binding of CPPs to a protein to carry the protein into the cell through the cell wall and lipid bilayer. Thus, we prepared ionic complexes of a CPP-containing carrier peptide and a cargo protein, namely, Citrine yellow fluorescent protein, and subsequently studied their physicochemical properties. Two types of carrier peptides, BP100(KH)9 and BP100CH7, were investigated for delivery efficiency into rice callus. Both BP100(KH)9 and BP100CH7 successfully introduced Citrine protein into rice callus cells under pressure and vacuum treatment. Moreover, delivery efficiency varied at different growth stages of rice callus; 5-day rice callus was a more efficient recipient for Citrine than 21-day callus.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Oryza / Proteínas de Bactérias / Portadores de Fármacos / Peptídeos Penetradores de Células / Proteínas Luminescentes Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Oryza / Proteínas de Bactérias / Portadores de Fármacos / Peptídeos Penetradores de Células / Proteínas Luminescentes Idioma: En Ano de publicação: 2019 Tipo de documento: Article