Experimental Protocol for Induction of Transgene Expression in Neural Stem Cells Through Polymeric Nanoparticles.
Methods Mol Biol
; 2125: 77-84, 2020.
Article
em En
| MEDLINE
| ID: mdl-31392588
ABSTRACT
Therapy based on stem cells utilizes these cells in neurodegeneration, brain/spinal cord injury, and much recently in repairing of severe heart diseases. Owning to their stemness, these cells are the potential source of progenitors that can offer a therapeutic remedy to a variety of diseases and/or disorders. The ability of these cells to regenerate and differentiate into specified phenotypes has great utility in tissue regeneration applications. This chapter provides a detailed account for isolation of neural stem cells from the mice embryo. Furthermore, the fabrication of chitosan-tripolyphosphate/hyaluronic acid-based nanoparticles and evaluating their efficiency in inducing transfection in the isolated neural stem cells as an approach for the treatment of neurodegenerative disorders.
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Base de dados:
MEDLINE
Assunto principal:
Polímeros
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Expressão Gênica
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Transgenes
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Nanotecnologia
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Nanopartículas
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Células-Tronco Neurais
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article